EFFECT OF ANTIOXIDANTS ON ALFALFA (MEDICAGO SATIVA L.) PROTOPLAST CULTURE AT EARLY STAGE
Qi Fang-jun Jia Jing-fen Li Ji-sheng (Laboratory of Cell Biology, Biology Department, Lanzhou University, Lanzhou 730000)
The present work revealed that the active oxygen radicals were generated from alfalfa callus cells during cell wall digestion in enzyme solution. By adding anti-oxidants into culture media, the protoplast division frequency was increased, and the browning of protoplast culture media was relieved to a certain extent. When the media was irradiated with ultra-violet rays prior inoculation of protoplasts, the proto- plast division frequency was reduced and the browning of medium became more serious. The negative effects of the irradiated medium on the protoplast culture could be overcome by supplementing antioxidants to the medium. These results suggested that the method of adding antioxidants to scavenge active oxygen radicals could improve plant protoplast culture condition at early stage.