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STUDY ON THE INFLUENCE OF ENZYMATIC DIGESTION UPON TUMOR-INFILTRATING LYMPHOCYTES

Li Biao-ru Tong Shan-qing Hu Buao-yu Zhu You-ming Zhang Xi-han Wu Jian-hua Lu Jing Lu De-yuan (Department of Microbiology, Shanghai Second Medical University)  
To get a long-term culture of tumor-infiltrating lymphocytes (TILs) is very difficult. The authors have investigated some suitable enzymes, their digestive conditions such as time and temperature, which may influence the viability and cyto-toxicity of TILs. The results showed that collagenase Ⅱ and Ⅳ could keep viability of TILs much longer than those treated with trypsin or hyaluronidase. The digestion with collagenase Ⅱ or Ⅳ at 4℃ for 24 hours was much less damage to viability of TILs than those treated at 37℃ for one hour. The TILs, which digested at 4℃ for 24 hours, still had cytotoxicity against autologus tumor cells as long as sixty to seventy-five days.
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