PLANTLET REGENERATION OF PROTOPLASTS DERIVED FROM CELL-SUSPENSION CULTURES OF OXYTROPIS LEPTOPHYLLA
Zhang Gen-fa (Biology Department, Henan Normal University, Xinxiang, 453002) Luo Xi-ming, Li Feng-xia and An Li-jia (Institute of Genetics and Cytology, Northeast Normal University, Changchun, 130024)
This paper reports the plantlets regenerated from protoplasts of Oxytropis lep-tophyllay. Morphogenic suspension cultures were first established from the calli initiated from plumular axis of donor plants. Protoplasts were isolated enzymatically from these suspension cultures and were purified through filtration and centrifugation. They were cultured in thin-layer liquid medium of modified K 8 P, supplemented with fluid medium CK 8.The minicalli formed through protoplast divisions were transferred into liquid medium S 12 for proliferation. When the morphogenic calli were formed, they were placed onto differentiation medium (NB + 2,4-D 0.01mg/L, KT 4.0mg/L) for the induction of shoots. After the roots were induced on the medium (1/2 MS + IBA 0.4mg/L, Suc. 2% ). the intact plant-lets were obtained.