Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Biologiae Experimentalis Sinica》 1994-01
Add to Favorite Get Latest Update

PLANTLET REGENERATION OF PROTOPLASTS DERIVED FROM CELL-SUSPENSION CULTURES OF OXYTROPIS LEPTOPHYLLA

Zhang Gen-fa (Biology Department, Henan Normal University, Xinxiang, 453002) Luo Xi-ming, Li Feng-xia and An Li-jia (Institute of Genetics and Cytology, Northeast Normal University, Changchun, 130024)  
This paper reports the plantlets regenerated from protoplasts of Oxytropis lep-tophyllay. Morphogenic suspension cultures were first established from the calli initiated from plumular axis of donor plants. Protoplasts were isolated enzymatically from these suspension cultures and were purified through filtration and centrifugation. They were cultured in thin-layer liquid medium of modified K 8 P, supplemented with fluid medium CK 8.The minicalli formed through protoplast divisions were transferred into liquid medium S 12 for proliferation. When the morphogenic calli were formed, they were placed onto differentiation medium (NB + 2,4-D 0.01mg/L, KT 4.0mg/L) for the induction of shoots. After the roots were induced on the medium (1/2 MS + IBA 0.4mg/L, Suc. 2% ). the intact plant-lets were obtained.
【CateGory Index】: S567.2;
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved