IDENTIFICATION OF IPs AND LAMINA IN CHLAMYDOMONAS SP
Fan Bin Li Yin-zhen Zhai Zhong-he (Department of biology, Peking University, Beijing 100871)
The intermediate filaments (IFs, 10nm diameter) network and nuclear lamina in unicellular green alga Chlamydomonas sp were demonstrated by means of selective extraction and DGD embedment-free electron microscopy. Immunoblot analysis, using monoclonal antibodies against animal keratins, showed the presence of keratin- like polypeptides (68 KD, 62 KD, 56 KD, 46 KD, 44 KD) in Chlamydomonas sp cells while polyclonal antibodies against mammalian lamins crossreacted with the total protein (101 KD, 90 KD). Indirect immuno-fluorescence microscopy gave similar results. These suggests that IFs and lamina existed in the Chlamydomonas cells.