HISTOCHEMICAL STUDIES ON A KIND OF INCLUSIONS IN INFECTED CELLS
Han Shan-hua Zhou Xiang-jun (Department of Sichuon Normal University, Chengdu 620066)
There is a kind of very special cyto-plasmic inclusions in soybean root nodules. They appear only in the infected cells which must be close on maturation or at maturation. In general, an infected cell only contains one inclusion. The inclusion locates in the outer portion of the infected cell and often near the intercellular space. The inclusions usually are round, their diameter is between 1-2 μm, sometimes even more than 2 μm. The inclusion is mainly made of granular material, tubular and vesicular element. There are abundant endoplasmic reticulum and dictyoso- mes around the inclusions. The inclusion is dark blue after staining with toluidine blue O, very black after staining with su-dan black B and blue after staining with Coomassie brilliant blue. Hitochemical studies show that the inclusion does not only contain lipid but also protein. Thus we consider the inclusion is not the massive ribonucleoprotein body, neither the usually protein body and lipid drop nor lipid body, it may be a kind of complex contained protein and lipid. Its functions may be related to the energy transposition and supply in symbiotic nitrogen fixation.