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HETEROTROPHIC TRANSFERATION AND LACTIC ACID FERMENTATION OF CHLORELLA PROTOTHECOIDES UNDER ANAEROBIC CONDITION

Huang Lu Wu Qing-yu (Department of Biology. Nanjing University, Nanjing 210008) Neil G. Grant (Department of Biology. The William Paterson College. NJ 07470, USA)  
The oxygen uptake rate in heterotro-phic growth Chlorella protothecoides was relative high. There was a peak of oxygen uptake respiration at the early log phase of the all growth period of the algal cells. It was shown by measuring the cell growth curve and variation of chlorophyll content in cells that the autotrophic algal cells could carry out heterotrophic trans-feration normally under aerobic condition, but could not complete heterotrophic trans-feration under anaerobic condition both in dark and in light. The algal cells which had completed heterotrophic transfera-tion also could grow slowly without oxygen and meanwhile the amount of lactic acid increased greatly both in cells and in medium. Most amount of lactic acid was released to the medium at the third day anaerobic growth of the cells. Heterotrophic transferation and lactic fermentation of Chlorella protothecoides had shown a good applied potential in micro-algal biotechnology.
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