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THE EFFECT OF SEVERAL FACTORS ON THE ASSEMBLY OF INTERMEDIATE FILAMENTS IN PLANT CELLS

Cheng Li-hong Zhai Zhong-he (Department of Biology, Peking University, Peking 100371)  
We prepared the intermediate filament proteins from carrot suspension cells using sequential extraction, dissolved them in 9 mol/L urea, removed the pellet by ultra-centrifugation, after dialysis the supernatant, it can assemble into 10 nm filaments; different factors have different effect on their assembly in vitro: (1) pH8.0 is the favourite pH for the assembly of interma- diate filament in plant cells; (2) the IF proteins also can assemble in phosphate buffer, but the filament is not as smooth and compact as the normal one; (3) Mg2+ and high ionic strength(150 mmol/L Nad) are good for the assembly of the filament, and also caused the aggregation of the filaments; (4) Ca2+ often caused the filaments uncompleted,seldom caused the obvious aggregation of the filaments.
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