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《Acta Biologiae Experimentalis Sinica》 1996-01
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STUDIES ON THE PHENOTYPE DEVIATION OF THE SINGLE-CELL SUBCLONES OF HUMAN STOMACH CARCINOMA MGC-803 CELL LINE

Han Ya-ling Zhang Zhi-qian Cai Xian-feng Zhang Wen-jun Lin Zhong-xiang (School of Oncology, Beijing Institute for Cancer Research, Beijing Medical University, Beijing 100034)  
The diversity of phenotypes of tumor cells is the main reason for the differences of drug-sensitivity, immunology, metastatic properties and cellular growth rate, and thus raises difficulties in tumor diagnosis and therapy. In order to investigate the mechanisms involved in the expression, correlation, development and regulation of the diversed malignant phenotypes, cell models from single-cell subclones of human stomach carcinoma cell line MGC-803 were studied. According to the growing time needed for the process of separation of each single-cell subclone, they were categoried into 3 groups as fast growing, moderatly and slowly growing subclones and named. From each group, representative subclones were selected——M17 fast, M 6 moderate and M3 slow, for further comparative studies. Foci frequency in soft agar was very high with M 17, very low with M 3, and M 6, the middle. The difference in the major transformed phenotype among the 3 subclones were statistically significant. There were differences in the function of gap junctional intercellular communication as detected by scrape-loading and dye transfer method; M 17 negative, while M 6 and M 3 were positive with dye transfer. Immunocytochemical staining of cytoskeleton elements showed heavy disruption of microtubule and microfilament networks and appearance of F-actin aggregates in M 17 cells. On the other hand, M 3 showed less disruptive changes and more normal appearance of microtubules and microfilament organization. Immunofluorescent staining showed marked differences in gene expression of c-myc, c-Ha-ras and c-met between M17 and M 3. M17 expressed intense fluorescence of the 3 onco-proteins while M 3 was negative with c-myc and c-Ha-ras, only weak c-met fluorescence in the cytoplasm.
【Fund】: 国家自然科学基金;; 北京市自然科学基金;; 北京市科技新星计划资助项目
【CateGory Index】: R735.2;
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