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《Acta Biologiae Experimentalis Sinica》 1996-04
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NUCLEAR MATRIX-LAMINA-INTERMEDIATE FILAMENT SYSTEM IN PTK 2 CELLS

JIAO Ren Jie CHEN Jian Ming ZHAI Zhong He (College oj Life Science, Peking University, Beijing 100871) YANG Lei CHEW En Ching (Department of Anatomy, The Chinese University of Hong Kong, Shatin NT, Hong Kong)  
Selective extraction, whole mount cell preparation and DGD embeddment-free section were involved in visualizing the nuclear matrix -lamina-intermediate filament system in PtK 2 cells. After extraction the anaphase chromosome residues adjoined to the cytoplasmic intermediate filament in some areas. Immunofluorescent staining showed us that the intermediate filament reacted with AE 1 and AE 3; McAb 223 could be localized specifically on the lamina while McAb C 23 could crossreact with cytoplasmic intermediate filament beside the lamina location, monoclonal antibody against lamin A (C) could also bind to chromosome residues. Antibody to 280 kD nuclear matrix protein which were positive stained in HeLa cells could not react with the nuclear matrix components of PtK 2 cells. 2-D elec-trophoresis demonstrated that there were some differences in the composition of the nuclear matrix-lamina-intermediate filament system of HeLa and PtK 2 cells. TdR treatment could lead to alteration of nuclear matrix proteins.
【CateGory Index】: Q24
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【References】
Chinese Journal Full-text Database 1 Hits
1 Zhai Zhonghe (College of Life Sciences, Peking University, Beijing 100871);NUCLEAR MATRIX-LAMINA-INTERMEDIATE FILAMENT SYSTEM[J];Bulletin of National Science Foundation of China;1998-02
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1 CHEN Wei-ying, WEN Bo-gui, ZHENG Rui-ming, WU Xian-ying, CHEN Ai-yun, WU Jian-yi, ZHANG Yan-mei (Department of Biochemistry, Medical College, Shantou University, Shantou 515041, China);Protecting effects of schisandrins on the nuclear matrix proteins and structure of hepatocyte in CCl_4-intoxicated rat[J];Immunological Journal;2006-04
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