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《Acta Biologiae Experimentalis Sinica》 1996-04
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XU Ling Fei GE Kai ZHENG Zhong Cheng SUN Lan Ying LIU Xin Yuan (Shanghai Institute of Biochemistry, Academia Sinica, Shanghai 200031)  
A negative selection system for glio-ma gene therapy was established in vitro. C 6 rat glioma cells were infected with recombined retrovirus which contain Es-cherichia coli cytosine deaminase (EC-CD) gene. The enzyme CD can transform the non-toxic prodrug 5-Fluorocytosine (5-FC) to the highly cellular toxic compound 5-Fluorouracil (5-FU). The growth inhibition studies proved that CD-positive cells were highly sensitive to 5-FC, the IC50 about 3 μmol/L, compared with an IC50 of approximately 6000μmol/L in parental C 6 cells. Both CD-positive and negative cells were sensitive to 5-FU at very low concentration (IC501μmol/L). Mixed cellular assay showed CD-positive cells had "bystander effect" on CD-negative cells when exposed to 5-FC. Our results demonstrate that EC-CD gene should be an efficient suicide gene for the treatment of glioma.
【Fund】: 国家“863”资助项目
【CateGory Index】: R739.4;
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