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《Acta Biologiae Experimentalis Sinica》 1996-04
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EXPERIMENTAL TREATMENT OF BRAIN TUMOR CELLS USING CD SUICIDE GENE

XU Ling Fei GE Kai ZHENG Zhong Cheng SUN Lan Ying LIU Xin Yuan (Shanghai Institute of Biochemistry, Academia Sinica, Shanghai 200031)  
A negative selection system for glio-ma gene therapy was established in vitro. C 6 rat glioma cells were infected with recombined retrovirus which contain Es-cherichia coli cytosine deaminase (EC-CD) gene. The enzyme CD can transform the non-toxic prodrug 5-Fluorocytosine (5-FC) to the highly cellular toxic compound 5-Fluorouracil (5-FU). The growth inhibition studies proved that CD-positive cells were highly sensitive to 5-FC, the IC50 about 3 μmol/L, compared with an IC50 of approximately 6000μmol/L in parental C 6 cells. Both CD-positive and negative cells were sensitive to 5-FU at very low concentration (IC501μmol/L). Mixed cellular assay showed CD-positive cells had "bystander effect" on CD-negative cells when exposed to 5-FC. Our results demonstrate that EC-CD gene should be an efficient suicide gene for the treatment of glioma.
【Fund】: 国家“863”资助项目
【CateGory Index】: R739.4;
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