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《Acta Biologiae Experimentalis Sinica》 1997-01
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STUDIES ON FORMATION OF THE MULTIPLE-BUDS AND ROOTING FROM SHOOT TIPS OF CAMELLIA RETICULATA L.IN VITRO

ZHUANG Cheng Ji ZHOU Jian Kui (Zhanjiang Ocean University, Zhanjiang 524025) (Kunming Institute of Botany, Academia Sinica, Kunming 650204)  
Effects of some factors on formation of the multiple-buds and rooting from shoot tips of Camellia reticulata L. in vitro have been studied. The results show that the kind and the concentration of hormone is the key which adjust formation of multiple-buds. The multiple-buds were formatted on the modified Murashi-ge and Skoog medium containing 3-5 mgL-1 bezylamino purine (BA) and 1-1.5 mgL-1 indoleacetic acid (IAA) or NAA. The formation of multiple-buds was obviously influenced by the age of plant from which explant was taken. The induction frequency of multiple- buds of explant taken from the seedling was higher than that from adult plant. The rejuvenate process of adult shoots was promoted markedly in subculture medium supplemented with coconut-milk (10%), allantion (1gL-1), Zeatin (1 mgL-1) and biotin (0.2-0.5 mgL-1). when using a "filter-paper bridge rooting method" for induction of rooting, it was found that the best rooting results were obtained by dip the basal ends of shoots in higher concentration (0.5 gL-1) of IBA or ABT solution for short periods (20 to 30 min.) then transferred the shoot onto a paper bridge on the MW liquid medium. The ability of producing root of the shoot was also influenced by the age of plant, the rooting rate and the number of roots per rooted shoot derived from the seedling were higher than that from adult plant. When the concentration of sucrose in rooting medium was 15 to 20 gL-1 the rooting rate could be marked risen. The experiment revealed that the rooting rate was evidently higher on MW or 1/2 ER medium than that on MS medium. The plant lets were transferred into soil where they grow normally.
【Fund】: 云南省科委课题
【CateGory Index】: S685;
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