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《Acta Biologiae Experimentalis Sinica》 1997-01
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ISOLATION AND SEQUENCING OF EMBRYOGENIC CELL PROTEIN FROM CARROT

ZHU Chang Fu KAMADA Hiroshi HE Yi Kun HE Meng Yuan HAO Shui (Institute of Genetics and Cytology, Northeast Normal University)  
Total soluble proteins were extracted from non-embryogenic cells, embryogenic cells and different developmental stage of somatic embryos from carrot (Daucus ca-rota L.) and were analyzed by isoelectric focusing sodium dodecylsulfate-polyacry-lamide gel electrophoresis(IEF/SDS-PAGE) two dimensional electrophoresis. The results demonstrated that two spots of 63-65 KDa molecular weight with pI ranging from 6.2-6.0 and two spots of 45 KDa molecular weight with pI ranging from 5.2-5.0, which were detected in embryogenic cells and undetected in non-embryogenic cells, were also detected in the extracts of different somatic embryos (globular stage,heart-shaped stage and torpedo-shaped stage). We concluded that some genes necessary for somatic embryo formation are already expressed in embryogenic cells. We successfully isolated and determined the partial amino acid sequences of ECP 45-2, a carrot embryogenic cell protein. The partial amino acid sequences of ECP45-2 show higher homology to ECP 45-1, which suggests that the genes for ECP45-1 and 45-2 are to be consist of a same gene family.
【CateGory Index】: Q946
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