Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Biologiae Experimentalis Sinica》 1997-01
Add to Favorite Get Latest Update

EXPRESSION OF DIPHTHERIA TOXIN A CHAIN GENE IN PLANTS COULD INHIBIT THEIR PROTEIN SYNTHESIS

WANG Jun (National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology, Academia Sinica, Shanghai 200031)  
The protein synthesis in protoplasts of Orychophoragmus violaceus could not be inhibited by expression of ricin A chain gene markedly even in the molecular ratio of CAT DNA and ricin A chain DNA more than 1:10. However, the protein synthesis in protoplasts of this plant and Nicotiana plumbagenifolia could be markedly inhibited by expression of diphtheria toxin A chain gene in protoplasts. The protein synthesis in the protoplasts of N. plumbagenifolia could be completely stopped in 2-3 hours after transfer of the fusion gene of dithpheria toxin A chain under control of CaMV 35 S promoter and Kozak sequence into protoplasts. During this period the protein synthesis in toxin gene-treated protoplasts is only about 5% of its activity in normal protoplasts .
【CateGory Index】: Q943
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Co-references】
Chinese Journal Full-text Database 1 Hits
1 Zhu Guo-feng, Qu Li-jia, Gu Hong-ya and Chen Zhang-Hang ( The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871);RECENT ADVANCES IN MOLECULAR BIOLOGICAL STUDIES ON DISEASE-RESISTANT GENES IN PLANTS[J];植物学报(英文版);1997-06
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved