ASSIGNMENT OF A NOVEL ZINC FINGER GENE ZNF191 TO HUMAN CHROMOSOME 18Q12.1 BY HUMAN/RODENT SOMATIC CELL HYBIRD PANEL AND FLUORESCENT IN SITU HYBRIDIZATION
SHI Shao Lin LIU Meng Mimg YU Long CHEN Shai Juan ZHENG Qi Ping WU Guo Jun CHEN Zhu ZHAO Shou Yuan (State Key Laboratory of Genetic Engineering ,Institute of Genetics, Fudan University ,Shanghai 200433,P. R. China) (Rui-jin Hospital ,Shanghai Second Medical University ,Shanghai 200025,P. R,China)
A novel human zinc finger gene, ZNF191,was assigned to chromosome 18 by hybridization of human/rodent hybrid cell panel to a full-length cD-NA as a probe. Meanwhile,a human genomic DNA lambda/DASH library was screened using this cD-NA probe and several positive clones were obtained. Fluorenscence in situ hybridization (FISH) was performed by using one of these positive clones,16-1,as a probe. Thus,the ZNF191 gene was precisely mapped in 18q12.1. To date, some hereditary diseases and tumors have been found to be associated with this region by analysis of genetic linkage and loss of heterozygosity. Hence, it suggested that the gene ZNF191 can be taken as a candidate gene responsible for those diseases and tumors.