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HE Ya Wen HAN Mei Li HE Hong ZHANG Lan Yin LI Geng Guang (South China Institute of Botany, Academia Sinica, Guangzhou 510650)  
Chinese cassava cultivars in vitro regeneration from 4 types of explants was studied. The results showed that thick shoots developed directly from the cut surfaces of "NZ 188" decapitated germination embryos on MS medium supplemented with 0. 05mg/L TIBA, 1.0mg/L BA with a regeneration frequency of 43%. When inoculated on MS medium with 0. 5mg/L NAA, 0. 5mg/L BA for 3 weeks, cotyledons from "SC 201" mature embryos produced shoots directly at a frequency of 42% , on MS medium with 0. 5mg/ L IBA, 1. 5mg/L BA, at a freuqency of 31%. Adding AgNO3 only or both AgNO3 and ABA to the media had an unfavorable effect on the shoot-induction. Calli could be induced when hypocotyl segments from "HZ 188" germination embryos were cultured on MS medium supplemented with 0. 5mg/L NAA, 0. 5mg/L BA in two weeks. After 4 weeks of culture on MS medium with 1mg/ L NAA, 2 mg/L BA green dots appeared on most calli, only 4. 4% calli produced shoots. Calli formed two weeks later when "NZ 188" stem segments were cultured on MS medium with 0. 05mg/L TIBA, 2mg/L BA. After 4 weeks of subculture only one callus produced shoots on the same medium.
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