IMMUNO-CYTOCHEMICAL LOCALIZATION OF MYOSIN IN BARLEY NUCLEI AND CHROMOSOMES
TAO Wei DUAN Xiao Gang WAN Li Hong ZHU Xiao Juan LUAN Yu Shi HE Meng Yuan (Institute of Genetic and Cytology, Northeast Normal University, Changchun 130024)
The nuclei and chromosomes of Hordeum vulgare were labeled by indirect immuno-fluorescence of anti-myosin antibody, after DNA, histone and part of non-histone proteins were removed. The results indicated that labeled fluorescence distributed all over the nuclei and chromosomes. By using the immunogold labeling technique, we further studied the distribution of myosin in nuclei and chromosomes, and revealed that a large number of the gold particles distributed evenly in the chromosomes. In the interphase nuclei, gold particles distributed mainly in nucleoli and chromatin. The above results showed that myosin is a constituent of the nuclei and chromosomes in barley.