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《Acta Biologiae Experimentalis Sinica》 1999-01
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ESTABLISHMENT OF STUDYING SYSTEM CLONED VERNALIZATION-PUTATIVE cDNA CLONE BY MEANS OF DIFFERENTIAL DISPLAY PCR

ZHAO Da Zhong CHEN Min TAN Ke Hui (Department of Cell Biology and Genetics,College of Life Sciences,Peking University,Beijing100871; Department of Developmental Biology,Institute of Botany,the Chinese Academy of Sciences,Beijing 100093)  
Differential display PCR has been developed as a tool detecting and characterizing specially expressed gene in eukaryotic cells recently. In this paper, a studying system was established which applied the novel approach of differential display PCR to clone vernalization-related cDNA clone. Here methodological details were provided which included the purification of total RNA,removal of contaminated DNA, reverse transcrip- tion of cDNAs, parameters of PCR, elec-trophoresis of amplified cDNAs, recovery and reamplification of cDNAs. It was also identified a vernalization-putative cDNA clone (VPC ) of VPC28 which was only expressed at the key stage of vernalization for 20 d in winter wheat. These results moreover made it readily applicable to a broad spectrum of similar studies.
【Fund】: 国家自然科学基金(批准号:39330010)~~
【CateGory Index】: S512.1;
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