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《Acta Biologiae Experimentalis Sinica》 2001-01
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1.4 kb 5′ FLANKING REGION OF CLASS I PATATIN DIRECTS TUBER-SPECIFIC GUS EXPRESSION IN POTATO (SOLANUM TUBEROSUM L.)

SONG Dong Guang ZHOU Jian Huang Da Qing Ma Huan SITU Ji Feng WANG Guang Qing WANG Xun Ming ( The State Key Laboratory of Geneticsand Genetic Engineering, Institute of Genetics, Fudan University, Shanghai 200433)  
Binary vectors pPATIs (with partial signal sequence) and pPATI (without signal sequence) were constructed by fusing 1.4 kb 5 flanking regions of Class I patatin gene with GUS. Transient GUS expression was observed in in vitro tuber slices bombarded with pPATI. These constructs were then introduced into potato (cv. Desiree) via Agrobacterium tumefaciens transformation. Trans-genie potato plants were confirmed by X-Gluc staining and PCR. Using in vitro tuberization system, GUS activities were assayed by fluorescence. It was shown that, in plants transformed with PATI-GUS, GUS specific aci-tivites were about 10 - 20 fold higher in tubers than in stems. Increased sucrose concentration could not induce PATI-GUS expression, but light enhanced PATI-GUS expreasion in cultured shoots.
【Fund】: 国家“863”高科技计划;; 国家自然科学基金(39700092);; 上海市高校科技发展基金~~
【CateGory Index】: S532;
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