KNOCKING OUT OF CEK2 AND CSK1 AFFECT THE PHENOTYPE OF CANDIDA ALBICANS
CHEN Jing CHEN Jiang Ye ( State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Acadarny of Sciences , Shanghai 200031, China )
We have cloned two novel mitogen-activated protein kinase (MAPK) genes CEK2 and CSK1 from Candida albicans strain SC5314. The heterozygous and homozy-gous cek2 and csk1 mutants were obtained by homologous recombination. The cek2 and csk1 null mutant strains CAJ3 and CAJ6 grew as wrinkled clonis on solid YPD medium. Expression of CEK2 gene in strain CAF3-1 repressed the filamentous growth of the strain weakly. Two-hybrid results showed Cek2 and Csk1 could not interact with Cph1 and Hst7 directly. Cek2 and Csk1 might cooperate with the filamentous transcriptional re-pressor Tupl.