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《Acta Biologiae Experimentalis Sinica》 2001-01
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BRCA1 IS DIFFERENTIALLY EXPRESSED IN HUMAN TUMOR CELLS

MENG Qing Hui YUAN Ren Qi Itzhak D. Goldberg Eliot M. Rosen Fan Sai jun ( Laboratory of Molecular Oncology Department of Radiation Oncology, Long Island Jewish Medical Center, The Long Island Campus for the Albert Einstein College of Medicine New York 11042, USA)  
Mutations of the human breast cancer susceptibility gene 1 ( BRCA1) confers a risk for breast, ovarian and prostate cancers and BRCA1 exerts multiple biological functions. Using Western blot and semi - quantitative reverse transcription - polymerase chain reaction ( RT -PCR) assays, we have determined the expression of endogenous BRCA1 protein and mRNA in forty - three human tumor cell lines established from eleven types of human tumor tissues. BRCA1 was differentially expressed in tumor cell lines. No significant association was found between BRCA1 expression and the p53 gene status of cell lines. The disruption of wild- type p53 by either the human papillomavirus E6 oncogene or the mutant p53 gene (143Ala→Val) did not cause any significant alteration in basal level of BRCA1 expression, while the knockout of p21 ( - /- ) by homologous recombination assay and Blocking Gadd45 expression by constitutive antisense expression slightly increased BRCA1 protein expression. Therefore, although the functional significance of the differential expression in human tumor cells is currently unknown, the present data provide a valuable background for further study of BRCA1 in tumor cell lines.
【CateGory Index】: R730.2;
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【Co-references】
Chinese Journal Full-text Database 5 Hits
1 HU Wen-Fang1,LIU Ming-Qiu2,ZHAO Qing1 1.Department of Obstetrics and Gynecology,Central Hospital of Huangshi City,Medical College,Wuhan University,Huangshi,Hubei,435000,P.R.China 2.Department of Pathology,Medical College,Wuhan University,Wuhan,Hubei,430071,P.R.China;Expression of P53,P63,and C-erbB-2 in Endometrioid Adenocarcinoma and Their Clinicopathological Significance[J];Chinese Journal of Cancer;2004-09
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5 Wang Jing, Du Chuanshu, Liu Tangbin, et al.  Dept. of Medical Genetics, Sun Yatsen University of Medical Sciences, Guangzhou 510089;Screening of Mutation of BRCA1 Gene in Wilm's Tumor[J];CHINESE JOURNAL OF PEDIATRIC SURGERY;1998-01
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