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《Acta Biologiae Experimentalis Sinica》 2001-01
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EFFECT OF α-LIPOIC ACID ON THE APOPTOSIS OF PC12 CELLS INDUCED BY 6-HYDROXYDOPAMINE

YUAN Chong Gang HE Ling XUE Xiao Lin SHI Ying Tang BI Xiu Hua (Department of Biology, East China Normal University, Shanghai 200062)  
PC12 cell line, a clonal cell line derived from a pheochromocytoma of rat adrenal medulla, was used as a model of dopaminergic neuron in vitro to study the effect of α-lipoic acid on the 6-OHDA induced apoptosis. The results from MTT method show that 6-OHDA decreased the cell survival rate obviously. Through TUNEL (TdT-mediated dUTP-biotion nick end labeling) and Flow cytometer (FCM) detection, we found that 6-OHDA triggered cell apoptosis and induced necrosis. It was confirmed by the different percentage of cell survival rate and apoptosis concluded from FCM and MTT. α-lipoic acid was used as antioxidant to protect the cell from 6- OHDA's injury . The result indicateed that α-lipoic acid can partly prevent apoptosis induced by 6-OHDA but fail to prevent necrosis since it can decrease the apoptotic cell from 20.09 % to 3.09 % , just as increased cell survival rate from 56.8 % to 72.6 % but can not reach the normal level showed by MTT assay. Biochemical approach showed the cell's antioxidant ability especial for SOD activity and GSH content increased after the treatment of α-lipoic acid. The data suggest that α-lipoic acid may protect PC12 cells from apoptosis induced by 6-OHDA through the antioxidant path.
【CateGory Index】: Q25
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