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《Acta Biologiae Experimentalis Sinica》 2001-01
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STUDIES ON ABA-INDUCED H_2O_2 IN Vicia GUARD CELLS BY MEANS OF CONFOCAL LASER SCANNING MICROSCOPY

ZHANG Xiao ZHANG Lin AN Guo Yong GAO Jun Feng SONG Chun Peng (College of Life Sciences, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100) ( Department of Biology, Henan University, Kaifeng 475001)  
The methods of confocal laser scanning microscopy (CLSM) and microinjection were used to study ABA-induced H2O2 in guard cells ( Vicia faba ), which were labeled with H2O2 specific probe-2, 7-dichlorofluorescin diacetate(H2DCFDA). The results indicated 100U/mL catalase (CAT) could inhibit partly stomatal clasure induced by ABA. 10-3mmol/L ABA could significantly induce H2O2 production in chloroplasl in guard cells of Vicia faba following microinjection, and 100U/mL CAT could partly abolish the effects following simultaneous microinjection of ABA and CAT. These suggest that H2O2 is possibly involved in ABA signaling leading to stomatal closure.
【Fund】: 国家自然科学基金(No.39870372);; 河南省自然科学基金(No.994012300)~~
【CateGory Index】: Q945
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