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《Acta Biologiae Experimentalis Sinica》 2001-01
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ZHANG Xiao ZHANG Lin AN Guo Yong GAO Jun Feng SONG Chun Peng (College of Life Sciences, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100) ( Department of Biology, Henan University, Kaifeng 475001)  
The methods of confocal laser scanning microscopy (CLSM) and microinjection were used to study ABA-induced H2O2 in guard cells ( Vicia faba ), which were labeled with H2O2 specific probe-2, 7-dichlorofluorescin diacetate(H2DCFDA). The results indicated 100U/mL catalase (CAT) could inhibit partly stomatal clasure induced by ABA. 10-3mmol/L ABA could significantly induce H2O2 production in chloroplasl in guard cells of Vicia faba following microinjection, and 100U/mL CAT could partly abolish the effects following simultaneous microinjection of ABA and CAT. These suggest that H2O2 is possibly involved in ABA signaling leading to stomatal closure.
【Fund】: 国家自然科学基金(No.39870372);; 河南省自然科学基金(No.994012300)~~
【CateGory Index】: Q945
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