MITOCHONDRIAL PERMEABILITY TRANSITION PORE REGULATES THE APOPTOSIS IN MGC-803 INDUCED BY THE EXTRACT OF GLYCYRRHIZA URALENSIS FISCH
MA Jing PANG Da Ben PENG Wen Lie LI Yu Mei XU An Long (Department of Biochemistry and Biopharmaceutical Centre, College of Life Science, Zhongshan University, Guangzhou 510275, P. R. China Institute of Fundamental Theory Research , China Academy of Chinese Traditional Medicine, Beijing 100700, P. R. China)
In our previous studies, we have discovered that the extract of glycyrrhiza uralensis Fisch ( EGUF) can induce obvious apoptosis in gastric cancer cell Line MGC-803. Here, further investigation was carried on about the time-lapse changes of mitochondria transmembrane potential, intracellular free calcium ions, DNA .electrophoresis, plasma membrane permeability and chromatin condensation during the apoptotic process of MGC-803 induced by EGUF and the influences of MPT-specific inhibitor Cyclosporin A(CsA) on these changes. Enhancement of plasma membrane permeability with PI staining, increase of intracellular free calcium ion and decrease of mitochondria transmembrane potential are early events in apoptotic cascades, prior to the appearances of apoptotic peak, chromatin condensation and DNA ladder. CsA significantly inhibited enhancement of plasma membrane permeability, change of intracellular free calcium ions and decrease of mitochondria transmembrane potential, also greatly delayed the progress of apoptosis. Thus, our results suggest that calcium and CsA-sensitive MPT is involved in the apoptosis of MGC-803 induced by EGUF.