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《Acta Biologiae Experimentalis Sinica》 2001-02
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OBSERVATION OF THE BIOLOGICAL CHARACRERIZATIONS OF NASOPHARYNGEAL EPITHELIAL CELLS BY EB VIRUS INFECTION IN EARLY PHASE OF IMMORTALIZATION

YANG Jing TANG Fa Qing GU Huan Hua DENG Xi Yun WENG Xin Xian TANG Min CAO Ya (Cancer Research Institute, Hunan Medical University, Changsha 410078)  
The multi-stage cell model of the nasopharyngeal carcinoma development in vitro by Epstein-Barr virus transformation is beneficial for the elucidation of the mechanism of nasopharyngeal cancer. To observe the biological changes of primary human nasopharyngeal epithelial cells in early phase of immortalization, in this study, we have detected the morphological changes and the expression profile of senescence-associated β-galactosidase (SA-β-Gal) in primary culture. In addition, the expression of EB virus latent membrane protein 1 (LMP1) and the growth curve of primary cells were also detected. Our results showed a low percentage of cells infected with EB virus expressing SA-β-Gal activity at the late primary culture. In morphology , the cells also formed multilayer foci, and the cell population doubling time was showed. These results demonstrated that the nasopharyngeal epithelial cells by EB virus infection have passed through the senescence and entered the early phase of immortalization. These cells have some of the transformed characteristics. Our results provided the data for further study on the mechanism of immortalization and the establishment of human nasopharyngeal epithelial cell line.
【Fund】: 国家重点基础研究发展规划(973)“鼻咽癌发生发展的基础研究”(项目编号:G1998051201);; 国家自然科学基金杰出青年基金(项目编号:39525022)资助
【CateGory Index】: R739.63;
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