MOLECULAR CLONING OF FULL LENGTH CAPSANTHIN/CAPSORUBIN SYNTHASE HOMOLOGOUS GENE FROM ORANGE (CITRUS SINENSIS)
XU Chang Jie CHEN Da Ming ZHANG Shang Long (Department of Horticulture, Huajiachi Campus, Zhejiang University, Hangzhou 310029)
The complete sequence of orange homologous capsan-thin/capsorubin synthase gene is 3788bp long with a coding sequence of 1512bp, which encodes a polypeptide of 503 amino acids. The 5' upstream sequence is 1721bp long and the 3' downstream sequence is 555bp long. The amino acid sequence of this gene is 78% and 69% identi- cal to the genes from carrot and pepper, respectively. It is also partially homologous to plant neoxanthin synthase, lycopene β-cyclase and lycopene ε cyclase genes. Isolation of the gene provides a framework for elucidation of the mechanisms involved in inability of citrus to produce cap-santhin and capsorubin.