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《Acta Biologiae Experimentalis Sinica》 2001-03
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EPSTEIN-BARR VIRUS LATENT MEMBRANE PROTEIN 1 INDUCES THE TELOMERASE ACTIVITY IN HUMAN NASOPHARYNGEAL EPITHELIAL CELLS

YANG Jing DENG Xi Yun GU Huan Hua XIA Lin Qing YI Wei CAO Ya (Cancer Research Institute, Hunan Medical University, Changsha Hunan 410078)  
Telomerase activation has been linked to cell immortalization in vitro and tumorigenicity in vivo. In this study, for the first, we reported that Epstein-Barr virus activated the telomerase activity of human nasopharyngeal epithelial cells in the early stage of immortalization as tested by the PCR-ELISA. The telomerase activity in na-sopharyngeal epithelial cells was only observed in pre-senescent cells. It was implicated that Epstein-Barr virus induced the escape of nasopharyngeal epithelial cells from senescence via the activation of telomerase. We further showed that telomerase activation in infected cells was de- pendent on the protein level of latent membrane protein 1 (LMP1) encoded by Epstein-Barr virus using a Tetracy-cline regulatory cell line expressing LMP1 , pTet-on-LMP1-HNE2. The activity of telomerase in nasopharyngeal cells was decreased when the protein level of LMP1 was blocked by antisense LMP1 plasrnid DNA. And the activity of telomerase was also related to the carboxyl terminus of LMP1. It was implicated that the ability of Epstein - Barr virus to suppress senescence is associated with telomerase activation by LMP1.
【Fund】: 国家重点基础研究发展规划(973)“鼻咽癌发生发展的基础研究”(项目编号:G1998051201);; 国家自然科学基金杰出青年基金(项目编号:39525022)
【CateGory Index】: Q51
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【References】
Chinese Journal Full-text Database 1 Hits
1 YANG Jing,DENG Xi-yun,DENG Lin,DIN G Lin,GU Huan-hua,YI Wei,CAO Ya(Cancer Research Institute,Hunan M edical University,Changsha 410078, China);Epstein-Barr Virus LMP1 Activates Human Telomerase Transcription via c-myc Expression[J];Chinese Journal of Virology;2003-03
【Co-references】
Chinese Journal Full-text Database 2 Hits
1 YANG Jing, DENG Xi Yun, TANG Min, WU Shang Hui, GU Huan Hua, YI Wei, CAO Ya ** (Cancer Research Institute, Xiangya Medical College, Central South University, Changsha 410078, China);Epstein-Barr Virus Latent Membrane Protein 1 Induces Telomerase Activity of Nasopharyngeal Epithelial Cells Through NFκB[J];Progress In Biochemistry and Biophysics;2002-04
2 LIAO Wei, YI Hong, LI Xiao Yan, TANG Ming, GU Huan Hua and CAO Ya * ( Cancer research Institute of Hunan Medical University, Changsha 410078, China );Doxycycline Dependent Expression of Epstein Barr Virus Latent Membrane Protein 1 with Tet Regulating System in Nasopharygeal Carcinoma Cell Line[J];ACTA BIOCHIMICA ET BIOPHYSICA SINICA;1999-03
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 ZHAO Tong-wei, CHEN Xiao-yi,LI Fei-hong,CAI Qiong-zhen (Department of Pathology, Guangdong Medical College, Zhanjiang 524023,China);Expressions of NF-κB, c-Myc and hTERT in benign and malignant nasopharyngeal diseases[J];Journal of Guangdong Medical College;2005-01
2 NAN Nan,ZHANG Wei-yuan(Department of Pathology,Guiyang Medical College,Guiyang 550004,China);The Significance of the Expression of c-myc,P21~(WAF1),LMP-1 and Its Relationship with the Expression of Ki67 in Nesopharyngeal Carcinoma[J];Journal of Guiyang Medical College;2006-01
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