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《Acta Biologiae Experimentalis Sinica》 2002-01
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ABERRANT DEVELOPMENT OF POLLEN IN TRANSGENIC TOBACCO EXPRESSING BACTERIAL iaaM GENE DRIVEN BY POLLEN-AND TAPE TUM-SPECIFIC PROMOTERS

NI Di An YU Xiao Hong WANG Ling Jian XU Zhi Hong (Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032; College of Life Sciences, Peking University, Beijing 100871)  
Microsporogenesis offers an ideal model for studying gene expression, cell division and cell to cell communication during development. The role of auxin in pollen development was investigated in transgenic tobacco plants expressing the coding region of the iaaM gene from Pseudomonas syringae, under control of the promoters Lat-52 (pollen-specific) or TA-29 (tapetum-specific). IAA level in anther of transgenic plants increased significantly, and transgenic plants displayed morphological aberrations not solely attributable to pollen development (such as adventitious root formation on stems, epinastic leaf growth, delayed flowering). These results suggest that expression of Lat-52 and TA-29 are not strictly limited to anther. Anther shape was changed and the number of pollen grains per anther was reduced, but grains could be stained with aceto-carmine. Almost all flowering plants were fertile, although the number of flowers per inflorescence was reduced compared with the wild-type ones. These results suggest that auxin plays an important role in pollen development, and over-expressing auxin synthesis gene could result in aberrant development of pollen.
【Fund】: 国家自然科学基金项目(39770079);; 植物分子遗传国家重点实验室的资助
【CateGory Index】: S572;
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