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《Acta Biologiae Experimentalis Sinica》 2002-01
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CHARACTERIZATION OF EXPRESSION OF THE CENTROSOMAL PROTEIN, CENEXIN, IN RAT SPERMATOGENESIS

SUN Xiao Dong YU Zuo Ren MA Jing GE Ye Hua LI Sai XUE She Pu HAN Dai SHu (Department of Cell Biology, Institute of Basic Medical Sciences, CAMS & PUMC, Beijing 100005)  
The centrosomal protein, Cenexin, is a molecular marker of mature centriole. To elucidate the variability and function of mature centriole in spermatogenesis, the high titer polyclonal antibody against rat cenexin was obtained by immunizing mice with recombinant cenexin which was made up in this study. The expression of cenexin in rat spermatogenesis was carried out by semi-quantitative RT-PCR, immunofluorescence and Western Blot. The results demonstrated that the level of Cenexin mRNA was higher in spermatogonia and spemiatocytes, then decreased in following stages, while Cenexin protein was located on one centriole from spermatogonia to spermatids, showing mature centriole existed in these stages. Cenexin protein was localized to the basal body of the flagellum in elongated spermatids and the stained signal disappeared in the most of epidy-dimal sperms. These results suggested that the expression pattern of cenexin in rat spermatogenesis might be related to the initiation of the flagella formation.
【Fund】: 国家科技部“973”资助[G19990559(01)]
【CateGory Index】: S814;
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【References】
Chinese Journal Full-text Database 1 Hits
1 YU Zuoren, GUAN Jikui, GE Yehua, MA Jing, GUO Rui, LI Sai, XUE Shepu & HAN DaishuDepartment of Cell Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China;Analysis of gene expression patterns with cDNA microarray during late stage of spermatogenesis in mice[J];科学通报(英文版);2002-24
【Co-references】
Chinese Journal Full-text Database 3 Hits
1 SUN Xiaodong , GE Yehua , MA Jing YU Zuoren LI Sai , WANG Yongchao XUE Shepu & HAN Daishu1. Department of Cell Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China;2. Key Laboratory of Cell Proliferation and Regulation, Department of Biology, Beijing Normal University, Beijing 100875, China;The cloning and expression characterization of the centrosome protein genes family (centrin genes) in rat testis[J];中国科学(C辑:生命科学)(英文版);2002-06
2 YU Zuo\|ren, SUN Xiao\|dong, GUAN Ji\|kui, XUE She\|pu, HAN Dai\|shu * (Department of Cell biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing\ 100005,China) [;SEPARATION AND PURIFICATION OF SPERMATOGONIA FROM CHANG-BAI BOAR TESTIS[J];Acta Anatomica Sinica;2002-06
3 SUN Xiaodong, MA Jing, GE Yehua, LI Sai, YU Zuoren, XUE Shepu & HAN DaishuDepartment of Cell Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China;Abnormal expression of cen-trosome protein (centrin) in spermatozoa of male human infertility[J];科学通报(英文版);2002-10
【Secondary References】
Chinese Journal Full-text Database 4 Hits
1 HUAI Ya-hong1,2,WANG Shu-hui1,XU Shang-zhong1,2,GAO Xue1,REN Hong-yan1,CHEN Jin-bao1(1.Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100094,China;2.College of Animal Science and Technology,Northwest A&F University,Yangling 712100,China);Progress on Studies of the Relative Genes on Sperm Quality[J];China Animal Husbandry & Veterinary Medicine;2008-03
2 Shi Bentao, He DalinDepartment of Urology, the First Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China(Shi BT, He DL);Genechip Technology Used in Basic and Clinical Male Infertility Research[J];Acto andrologica Sinica;2004-04
3 Shao-Qin GE Fei DUAN Xian-Jiang KANG1 Meng-Ran ZHAO1(Hebei University Health Science Center,Baoding 071002,China;1 College of Life Sciences,Hebei University,Baoding 071002,China);The acetylation of histone H4 during spermatogenesis[J];Chemistry of Life;2009-02
4 GE Shao-Qin1, 2, KANG Xian-Jiang1, LIU Gui-Rong1, MU Shu-Mei1 1. College of Life Science, Hebei University, Baoding 071002, China;2. Hebei University Health Science Center, Baoding 071002, China;Genes involved in spermatogenesis[J];Hereditas;2008-01
China Proceedings of conference Full-text Database 1 Hits
1 DONG Wu-Zi, ZHUANG Shu-Zhen, DOU Zhong-Ying (1.Shaanxi Province Branch Center of National Stem Cell Engineering & Technology Center, Northwest Sci-tech University of Agriculture and Forestry, Wangling 712100, China;);Proceeding of Study on Meiosis of Male Germ-line Stem Cells[A];[C];2004
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