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《Acta Biologiae Experimentalis Sinica》 2002-01
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THE ULTRACYTOCHEMICAL LOCALIZATION OF ATPase ACTIVITY IN POLLEN TUBE AND STIGMA OF FAGOPYRUM ESCULENTUM AFTER COMPATIBLE AND INCOMPATIBLE POLLINATION

GAO Xin Qi WANG Xiu Ling (Department of Biology, Qufu Normal University, Shandong Qufu 273165)  
Ultracytochemical localization of adenosine triphosphatase(ATPase) activity in stigmas, pollens and pollen tubes of Fagopyrum esculentum was perfeimed with the cytochemical method of lead phosphate precipitation. The results were as follows: (l)Lower activities of ATPase appeared in stigma cells at 0. 5 hour after compatible and incompatible pollination. Stigma surface and pollen grains attached on stigma showed higher ATPase activities after compatible pollination and lower or no activities after incompatible pollination at 0. 5 hour. ATPase localized on endoplasmic reticula and sperm cell in pollen grain. (2)Lower ATPase activities appeared both in stigma cells and pollen tubes in style at 1. 5 hours after incompatible pollination. Pollen tube stopped growing and the degeneration of its cytoplasma began; on the contrary, at 1. 5 hours after compatible pollination,higher ATPase activities were detected both in stigma cells and pollen tube in style. ATPase localized mainly on plasmolemma, in cytoplasmic matrix of stigma cells and in mitochondria, dictyosome, plastid envelop, and on the wall of pollen tube as well. The present study indicated that the stop of pollen tube growth after self-incompatibility of Fagopyrum esculentum resulted not only from the nutrient starvation of pollen tube, but also from its metabolic disorder in incompatible style.
【CateGory Index】: S517;
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【References】
Chinese Journal Full-text Database 1 Hits
1 Xin-Qi Gao1,2*( 1Collage of Life Science, Qufu Normal University, Qufu 273165, China;2State key Laboratory of Plant Physiology and Biochemistry, China Agricultural University, Beijing 100094, China );Transportation and Accumulation of Storage Protein and Regulation of Genes Expression in Seeds[J];Chinese Journal of Coll Biology;2005-01
【Co-references】
Chinese Journal Full-text Database 7 Hits
1 CHEN Qing-Fu [WT8.5BZ]( Laboratory of Genetics,Biology Department, Guizhou Normal University, Guiyang 550001, China );Karyotype analysis of five Fagopyrum species native to China[J];Guihaia;2001-02
2 GAO Xin qi WANG Xiu ling (Department of Biology, Qufu Normal University, Qufu 273165);THE AGGREGATE ROUGH ENDOPLASMIC RETICULA IN COTYLEDON DURING THE DEVELOPMENT OF BUCKWHEAT EMBRYO[J];Bulletin of Botanical Research;2002-01
3 ZHAO Xiao-zhen,ZHANG Zheng,JING Wei,LI Yu-ying,WANG Zhuan-hua* (Key Laboratory of Chemical Biology and Molecular Engineering, Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China);Cloning and Sequence Analysis of N-terminal Gene of Major Allergenic Protein in Tartary Buckwheat[J];Food Science;2006-10
4 WANG Lan, LI Yu-Ying, CAI Gui-Hong, ZHANG Zheng, WANG Zhuan-Hua~*(Key Laboratory for Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China);Prokaryotic Expression and Immunological Identification of Tartary Buckwheat Allergenic Protein (TBa)[J];Chinese Journal of Biochemistry and Molecular Biology;2006-04
5 ZHANG Chao,GUO Guan- xin,ZHANG Hui (Food College of Jiangnan University, Wuxi Jiangsu 214036);Extracting Processing Study of Buckwheat Protein[J];;2004-01
6 GAO Xin-Qi1,XI Xiang-Yuan 2* (1 Department of biology, Qufu Normal University, Shandong 273165,China; 2 College of Life Sciences, Shandong Agriculture University, Shandong 271018,China);The Development of Embryo and Endosperm and the Accumulation of Storage Reserves in Fagopyrum esculentum[J];Acta Botanica Yunnanica;2000-04
7 Zhu Shengtao, Wu Kun (Public Health School of Harbin Medical University, Harbin);NUTRITIONAL EVALUATION OF PROTEIN——Ratio Coefficient of Amino Acid[J];Acta Nutrimenta Sinica;1988-02
【Secondary References】
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1 XIAO Gang,ZHANG Xiu-ying,ZHANG Zhen-qian,GUAN Chun-yun(Oil Crops Institute,HNAU,Changsha 410128,China);Construction of subtractive library for differentially-expressed genes at different developmental stages in Brassica napus[J];Journal of Hunan Agricultural University(Natural Sciences);2008-05
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