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《Acta Biologiae Experimentalis Sinica》 2002-01
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ULTRASTRUCTURAL LOCALIZATION OF TRANSCRIPTION SITES OF rRNA GENES IN THE NUCLEOLUS OF WHEAT

HE Jie TAO Wei (Department of Biotechnology, Harbin Institute of Technology, Harbin 150001) (College of Life Sciences, Peking University, Beijing 100871)  
There were no definite conclusions about transcription sites of rRNA genes in nucleoli of eukary-otes for a long time. We used the wheat as specimen and observed ultrastructure of chromatin in FC of nucleoli by conventional transmission electron microscope. And we employed anti-DNA antibodies to analyze the distribution of DNA in nucleoli, and found that DNA located in FC, DFC and the transitional region between them. Using anti-UBF antibodies to study localization of RNA polymerase I transcription factor UBF in nucleoli, we observed that UBF located in DFC and the transitional region between DFC and FC in wheat, but not in FC. Furthermore, with anti-RNA/DNA hybrid antibodies, we directly and selectively labeled the transcription sites of rRNA genes of nucleoli and revealed that it located in DFC and the transitional region between DFC and FC in wheat.
【Fund】: 国家重点基础研究发展规划(973计划)资助项目(项目编号J1999053902)
【CateGory Index】: S512.1;
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【References】
Chinese Journal Full-text Database 1 Hits
1 HE Jie 1 ,TAO Wei 2 ,HAO Shui 3 1.Department of Life Science and Engineering,Harbin Institute of Technology,Harbin 150001,China; 2.College of Life Sciences,Peking University,Beijing 100871,China; 3.Institute of Genetics and Cytology,Northeast Normal University,Changchun 130024,China;Localization of nucleolar DNA and transcription sites of rRNA genes in situ in wheat cells[J];Hereditas;2008-02
【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 TAO Wei JIAO Mingda HE Jie HE Mengyuan HAO Shui Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China Correspondence should be addressed to Hao Shui Current address: School of Life Science, Peking University, Beijing 100871, China;Structures of nucleolus and transcription sites of rRNA genes in rat liver cells[J];中国科学(C辑:生命科学)(英文版);2000-03
2 TAO Wei, HE Jie, JIAO Mingda, HE Mengyuan & HAO ShuiDepartment of Cell Biology, College of Life Sciences, Peking University, Beijing 100871, China; Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China;Configuration of nucleolar DNA in situ in nucleolus of Allium cepa cells[J];科学通报(英文版);2001-01
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