CONSTRUCTION OF SHUTTLE EXPRESSION VECTOR AND EXPRESSION OF THYMOSIN α_1 IN SYNECHOCOCCUS SP.PCC7942
ZHANG Jun QIN Yan OUYANG Qing XU Hong ZHOU Ke Fu LIU Ren Hai LOU Shi Lin (The Key Laboratory of Ministry of Education for Cell Biology and Tumor Ce/l Engineering School of Life Science, Xiamen University, Xiamen 361005)
The shutter expression vector pPREUT was constructed from the plasmid pPRS-1 containing the en dogenous snail plasmid of Plectonema boryanum. The expression elements such as heat shock gene groESL promoter, foreign gene Ub-thymosin α1, rbcS polyA terminator and Kanamycin resistance gene were all included. The shutter plasmid pPREUT was direcdy transferred into Synechococcus sp. PCC7942. The transformants were obtained through Kanamycin screening. Southern blotting analysis showed that the shutter plasmid have been transferred into Synechococcus sp. PCC7942. After induction by heat shock(42℃) for 30 min, the foreign protein LIR-Tα1 was ex pressed efficiently, which reacted 7.5% of total amount protein.