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A SIMPLE AND HIGHLY EFFICIENT AGROBACTERIUM -MEDIATED RICE TRANSFORMATION SYSTEM

LI Mei Ru LI Hong Qing ( South China Institute of Botany, The Chinese Academy of Sciences , Guangzhou 510050; College of Life Sciences,South China Normal Umversity, Guangzhou 510031)  
A simple and highly efficient rice transformation system was established based on the studying of lactors influencing the Agrobacteritnn -mediated rice transformation. Embryogenic calli derived from mature embryos were infected and cocultivated with A . tumejaciens EHA101 harboring binary vectors:pHQ9,pHQ10,pHQT3. The highest transformation frequency was about JOO bypromycin resistance calli per gram of calli explants,and above 85% of these calli could be regenerated into plants. The putative transgenic plants were confirmed by GUS assay and southern-blot analysis. Hygromycin resistance tests indicated that the segregation of transgene in T1 progeny corresponded to the Mendelian ratio,3:1. This system will benefit the functional genomic study of rice by using T-DNA insertion mutagenesis and gene targeting.
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