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WANG Yun Shan SHEN Hong SUN Shan Hui JIANG Li Hua LIU Yang ZHU Zhi Wei XIAO Dong Jie HUANG Ping YANG Bo DU Xi Yan ZHANG Yuan Chao ( Centrol Laboratory, Jinan Centrol Hospital, Clinmal Medical College of Shandong University Jinan 250013; Department of Rheumatology, Shandong People's Hospital, Jinan 250021)  
To discuss the fasle-positive of serological diagnostic testing for coronavirus antibody in patients with systemic lupus erythematosus(SLE), 66 normal individual and 31 SLE with non-SARS patients were detected for SARS-associated coronavirus (SARS-CoV) antibody and RNA by enzyme-linked immunosorbent assays(ELISA) and reverse transcriptase-polymerase chain reaction(RT-PCR). The result showed 2/66 cases(3. 0%) were positive of SARS-CoV-IgG antibody and 66 cases were negative of SARS-CoV-IgM antibody in the 66 cases healthy controls; in 31 cases with SLE, positive rates of SARS-CoV-IgG and IgM antibody were 58. 1 % ( 18/31 ) and 29% (9/31), respectively, in which 7 cases(22. 6% ) were positive of both SARS-CoV-IgG and IgM antibody. All samples of positive SARS-CoV-IgG and IgM antibody were negative by RT-FCR. The ELISA kit coated by non-purification antigen may induce the false-positive of SARS-CoV antibody in patients with SLE. This result suggested that the specificity of ELISA tests for SARS was excellent and has low fasle-positive rates when using SARS-CoV-IgG and IgM antibody tests .A possible cause of false-positive of SARS-CoV-IgG and IgM antibody in SLE patients is coated antigens with SARS-CoV and Vero-E6 cells in ELISA methods.
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