TISSUE CULTURE AND PLANT REGENERATION OF HELIANTHEMUM SONGARICUM SCHRENK
LI Jing JIA Jing fen HAO Jian guo (School of Life Science , Northwest University, Xi an 710069)
The cotyledonary segments of sterile seedlings of Helianthemum Songaricum Schrenk were cultured on different media containing different phytohormones. It was found that the calli could be induced efficiently on MS basal medium supplemented with 10. 0mg/L NAA and 0. 2mg/L 6-BA . When calli were transferred on MS medium with 2. 0mg/L 6-BA and 0. 2mg/L NAA, shoots were produced. The frequency of shoot differentiation reached about 85%. The regenerated shoots were rooted on 1 /2MS medium added with 0. 5mg/L NAA. The rooting rate was about 76% . Regenerated plantlets were successfully transplanted in soil, with a success rate of 67% .