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《Acta Biologiae Experimentalis Sinica》 2004-01
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MA Suo Feng LUO Ming Jiu MIAO Yi Liang LAN Guo Cheng WANG Zi Yu LIU Xin Yong CHANG Zhong Le TAN Jing He (College of Animal Science and Technology, Shandong Agriculture University, Taian 271018)  
The meiotic progression of goat oocytes from follicles of different diameters was investigated in this study. The results were summarized as follows: (1) The in vitro meiotic maturation capacity was different among oocytes from follicles of different diameters. And thus oocytes from ≤ 0.5mm follicles were unable to resume meiosis; oocytes from 0.8-1.2mm follicles were capable to resume meiosis, but could develop only to M I stage (60% at 24 h); oocytes from 1.5-5mm follicles had acquired full-meiotic maturation capacity and 91% of them developed to M Ⅱ stage at 24 h of culture. (2) The percentage of oocytes with intact-germinal vesicles from 1.5-5mm follicles decreased significantly during 2-8 h of in vitro maturation and the decrease was even more rapid during 4-6 h of culture (from 60% to 19%, p0.0005). The percentage of oocytes at M I-stage increased from 24% to 61% during 6-12 h of in vitro maturation, and it then decreased. By 24 h of culture, only 2% oocytes remained at M Ⅰ-stage. Twenty one percent of the oocytes in this group developed to MⅡ-stage at 16 h of culture, and by 24 h of culture, 91% were at MⅡ-stage. (3) Statistic analysis of the meiotic progression (the duration of each cell cycle stage) of oocytes from 1.5 -5mm follicles showed that GV stage lasted from 0 to 3 h of culture, prometaphase-Ⅰ stage was from 3.0 to 7.0 h, metaphase-Ⅰ stage was from 7.0 to 14.6 h, anaphase-Ⅰ / telophase-Ⅰ was from 14.6 to 18.4 h and metaphase-Ⅱ stage lasted from 18.4 to 24 h. (4) Whether the oocytes capable of GVBD and entrance of M I developed to M Ⅱ, the timing of meiotic progression prior to M I was similar. In summary, our results provided necessary data for studies on the mechanisms and control of meiosis in mammalian oocytes.
【Fund】: 国家科技部“973”项目(编号G200016107)
【CateGory Index】: Q343
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1 SHI QI-XIAN ZHONG CUI-LING(Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310013)CHEN DA-YUAN(Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080)XU ZHI(Tianjin Institute of Husbandry & Veterinary Science, Tianjin 300112);IN VITRO CAPACITATION OF RAM SPERM[J];Acta Zoologica Sinica;1991-01
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6 WU Yan Guang YU Yuan Song TAN Jing He (College of Veterinary and Animal Sciences, Shandong Agricultural University Taian 271018, China);Follicular Dynamics and its Regulation in Mammals[J];Chinese Journal of Zoology;2002-06
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【Secondary References】
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1 Wang Xuehong Dong Yajuan* Bai Xuejin* Yue Fujie Yang Shuling Dong Fangyuan(The Center of Animal Embryo Engineering Laiyang Agricultural University, Qing Dao Shandong 266109);Study on Germinal Vesicle Migration of Goat During IVM and IVF[J];Biotechnology Bulletin;2006-S1
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