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《Acta Biologiae Experimentalis Sinica》 2004-01
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COMPARATIVE STUDIES ON PROTEINS OF CYTOPLASMIC MALE-STERILE WHEAT AND ITS MAINTAINER BY 2D-PAGE IN TRITICUM AESTIVUM

FAN Bao Li WANG Zhen Ying CHEN Hong PENG Yong Kang (Department of Biology,Tianjin Normal University, Tianjin 300074)  
By using 2D-PAGE techniques, protein compositions have been studied at different developmental stage leaf (seedling, tillering, shooting and booting stages) and pollen mother cells (PMC)(meiotic, 1-nucleus and 2-3 nuclei stages)in male sterile wheat and its maintainer. The results indicated that a specific protein (33KD/pI6.3) identified at the shooting and booting stage leaves in male-sterile wheat, but this protein species has not been identified in the male fertile wheat. Four protein species, 53KD/PI5.5, 50KD/PI5.7, 48KD/PI5.6, 20KD/PI7.5 were detected at the 2-3 nuclei stages,which was thought key stage of pollen fertility lost in male-sterile wheat, and these protein species were not be seen in male-fertile wheat. According above experiment results, five protein species which detected in leaf and PMC might participate in fertility regulation and related to the male-sterility of male-sterile wheat.
【Fund】: 教育部科学技术研究重点项目(02010)
【CateGory Index】: S512.1;
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