PROTECTIVE EFFECT OF ORGNOSELENIUM FROM Se-ENRICHED LACTOBACILLUS ON LIPID PEROXIDATION REACTION AND NK CELL ACTIVITY IN SPLEEN OF LIVER INJURY MICE
CHEN Long;ZHU Shan Liang;CAO Meng College of Life Sciences, Nanjing Normal University; Jiangsu Engineering Research Center for Bio-medical Function Materials, Nanjing 210097, China
Changes of lipid peroxidation reaction and NK cell activity in spleen of CCl_4-in- duced liver injury mice with adding orgnoselenium from Se-enriched lactobacillus were studied to discuss the protective effect of orgnoselenium and its mechanism. Sixty healthy mice (female: male=1:1) were chosen and divided randomly into four groups: control group(group C), orgnoselenium group (group Se), CCl_4-injection group (group CCl_4) and CCl_4-injection plus orgnoselenium group (group CCl_4-Se). The liver injury was induced by abdominal injection of CCl_4 (0.07ml/100g body weight) on every other day over four weeks. The spleens were collected at the 2nd and 4th week, and spleen NK cell activity,spleen homogenate GSH-Px, SOD, CAT activities and MDA concen- tration were determined. The results showed that during the entire experimental period, spleen homogenate GSH-Px, SOD and CAT activities in groups C, Se and CCl_4-Se were higher or sig- nificantly higher than that in group CCl_4, and three antioxidant enzyme activities in groups Se and CCl_4-Se had no apparent differences from that in group C except that there were significant in- creases of SOD activity at the 4th week. Spleen homogenate MDA content of group CCl_4 in- creased markedly compared with that of groups C, Se and CCl_4-Se, MDA level of group CCl_4-Se was close to that of group C, and that of group Se was lower. During the entire experimental peri- od, NK cell activity of group Se was the highest and significantly higher than that of group C at the 4th week, a lowest value was observed in group CCl_4, which was lower or markedly lower than that of groups C, Se and CCl_4-Se, there were no significant differences between group CCl_4- Se and group C. It is suggested that orgnoselenium from Se-enriched lactobacillus can enhance antioxidation ability in normal mice and play an effective role by means of improving and enhanc- ing the spleen antioxidation enzymes and NK cell activities in the process of intervening liver in- jury.
【Fund】： 江苏省重点实验室开放基金（No．KJS01072） 江苏省生物医药功能材料工程研究中心资助~~
【CateGory Index】： R151;
【CateGory Index】： R151;