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《Journal of Molecular Cell Biology》 2007-01
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STUDIES OF DYNAMIC DISTRIBUTION OF Thr11-PHOSPHO-RYLATED H3 IN MCF-7 AT MITOSIS

ZHOU Hao LI Deng Wen SONG Li Ping LIU Ru Ming CHEN Jia Tong HUANG Xi Tai (Department of Biochemisty and Molecular Biology,Nankai University,Tianjin 300071)  
The phosphorylations of histone H3 on Ser10,Ser28,Thr11 and Thr3 of the amino terminal are the events that related to mitosis of the cell.To study the function of Thr11 phosphorylation of histone H3,indireet immunofluorescence labeling and laser confocal were employed with the antibody that was specific for Thr11-phosphorylated histone H3.The celluar dynamic distribution of this protein was examined at mitosis in MCF-7 cell.Our results showed that Thr11-phosphorylation of H3 initiated at centromere at early prophase in MCF-7 cells.The fluorescence signal on Thr11-phorphoslated histone H3 reached the strongest at early metaphase at the centromere punctated in the central of mitotic cell.The dephosphorylation of Thr11- phorphoslated histone H3 was completed in anaphase.The behavior of Thr11 phosphorylation and dephosphorylation in mitotic cell of MCF-7 were different from that of Ser10 phorphorylation and dephosphorylation.The results suggest that the Thr11 phosphorylation of histone H3 has a specific function in mitosis different from Ser10 phosphorylation.There was a precise spatial and temporal correlation between H3 phosphorylation of Thr11 and initial stages of chromatin condensation. Thr11-phorphoslated histone H3 located on centromeres suggest that it maybe included in the active kinetochore during mitosis.
【Fund】: 本项目由南开大学创新基金资助。
【CateGory Index】: Q253
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