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《Journal of Molecular Cell Biology》 2007-01
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ZHANG Huai Yu~(1,2) REN Zheng Long~(2**) (~1College of Life Sicence,Sichuan Agricultural University,Ya'an Sichuan 625014; ~2State Key Laboratory of Plant Breeding and Genetics,Sichuan Agricultural University,Ya'an Sichuan 625014,China)  
Weiling rye(S.cereale L.cv.),a Chinese dwarf rye,confers high powdery mildew (Erysiphe gramininis f.sp.tritici)in China.My8443,a wheat cultivars infecting seriously powdery mildew disease,was used as the female parent and Weiling rye was used as the donor of powdery mildew resistance in the study.A new wheat-rye translocation line,named No.147,was developed from BC2F6 progenies of wheat cultivars My8443 and Weiling rye to transfer the resistance from Weiling rye to common wheat.The powdery mildew resistance of No.147 and its parents were in- vestigated in seedling and adult stages by artificially inoculating the mixture of advanced pathogenic races in room and field and the single pathogenic race in room.Improved Giemsa C- banding technique and genomic in situ hybridization(GISH,Genomic in situ hybridization)were used to identify wheat and rye chromosomes.Acid polyacylamide gel electrophoresis(APAGE)sep- aration of endosperm gliadin and simple sequence repeat(SSR)PCR amplification of 11 SCM-Se- cole cereale markers also were employed for 1RS confirmation in the study.The results showed that No.147 was a new 1BL/1RS wheat-rye chromosome translocation with high powdery mildew resistance derived from Weiling rye.The reason on the formation of the new wheat-rye chromo- some translocation was analyzed.The utilizations of resistance gene resource derived from Chinese Weiling rye and the new 1BL/1RS translocation line in wheat genetics and breeding improvement were discussed in the paper.
【Fund】: This work was supported by National Natural Sciences Foundation of China(No.30170579);; the Program to Innovative Research Team in University(No.IRT0453);; the Theory and Apply Program of Sichuan Province Science and Technology Bureau(No.03NG002-008);; the Key Program of Siehuan Province Education Bureau(2004A020).
【CateGory Index】: S512.1
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