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《Journal of Molecular Cell Biology》 2007-01
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THE DEVELOPMENT OF SECONDARY CELLS IN THE CALLUS OF HYPERICUM PERFORATUM L.AND HYPERICINS ACCUMULATION

SONG Xin~1 ZHU Jian~1 LV Hong Fei~2 (~1School of Life Science and Technology,Tongji University,Shanghai 200092,China; ~2College of Life and Environment Science Zhejiang Normal University,Jinhua 321004,China)  
Hypericum perforatum L.is a kind of traditional herbal medicine that has been used as an anti-depression medicine in Europe for centuries.One of its biological active com- pound,hypericins,is stored in the special secondary structure,black nodules,which located in the stems,leaves and flowers.Most researches focus on the development of the black nodules in vivo and how to culture the plant to produce more hypericins.We studied the process of de-differentia- tion from explants to callus and the pathway of hypericins biosynthesis in callus and cells of H. perforatum L.which reflected the relationship between the cell development and secondary metabo- lites accumulation.The morphogenesis of cells development and hypericins accumulation were stud- ied by electron microscopy and histological technologies. Hypericins began to accumulate in a bunch of secondary ceils located on the surface of the callus in late development period.Hypericins initially produced in the cytoplasm and were trans- ported into the vacuole and then accumulated.E.R.took apart in the process of hypericins pro- duction.Theses results supplied the gap of hypericins production and accumulation in vitro and gave some useful information regarding mass-production hypericins by tissue and cell culture tech- nology.
【Fund】: 国家自然科学基金资助(30470863)。
【CateGory Index】: S567.19
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