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《Journal of Molecular Cell Biology》 2007-01
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COMPARISONS AMONG DIFFERENT METHODS OF CULTURING NEURAL STEM CELLS ISOLATED FROM HUMAN FETAL CORTEX

REN Ping~(1,2) GUAN Yun Qian~(1,2) ZHANG Yu~(1,2*) (~1Cell Therapy Center,Xuanwu Hospital,Capital University of Medical Science,Beijing 100053; ~2Key laboratory of Neurodegeneration,Ministry of Education,Beijing 100053,China)  
Neural stem cells(NSCs)are proved to be promising cell sources for gene therapy and cell therapy.To pursue optimal conditions for the isolation and culture of neural stem cells residing in human fetal cortex,the cortical tissue was dissociated mechanically and digested with various enzymes.Short-term trypsin digestion combined with pipette dissociation proved to be the suitable method of isolating human NSCs derived from the fetal cortex.Furthermore,DMEM/F12 medium was superior to the neurobasal medium in the aspect of clonal formation.In repeated dissociation experiments,it was found that accutase,instead of trypsin,endowed the NSCs with better growth and efficient neurosphere formation.
【Fund】: 本项目得到国家自然科学基金(30428010);; 国家973课题(001CB510104);; 教育部新世纪优秀人才项目资助。
【CateGory Index】: Q813.1
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