ANALYSIS OF GENETIC DIVERSITY AMONG 13 CHINESE SPECIES OF DENDROBIUM BASED ON AFLP
WANG Hui Zhong1 LU Jiang Jie2 SHI Nong Nong1 ZHAO Yan3 YING Qi Cai1 (1Key Laboratory of Hangzhou City for Biochemistry and Molecular Biology,Hangzhou Normal University,Hangzhou 310018, China; 2Zhejiang Forestry College, Provincial Key Lab for Modern Silvicultural Technology, Linan 311300, China; 3College of Science Biotechnology and Environmental Engineering, Zhejiang Gongshang University, Hangzhou 310018, China)
Amplified fragment length polymorphism(AFLP) was assessed in 13 Chinese species of Dendrobium. Three primer combinations E+ACT/M+CAC, E+AAC/M+CAC and E+ACA/M+CAC were used for selective amplification. Between 100bp and 300bp, a totle of 346 fragments were detected, in which 342 are polymorphic. The rate of polymorphism was 98.8%. The AFLP data were analyzed using Nei Li similarity coefficient and UPGMA Cluster method. The result indicated that the 13 species were clustered into three groups on coefficient level 0.54. GroupⅠincluded D. falconeri,D. officinale,D. wilsonii, D. hercoglossum, D. crystallinum. D. hancockii, D. guangxiense, D. primulinum, D. crepidatum,D. thyrsiflorum. GroupⅡincluded D. chrysotoxum. Group Ⅲ included D. loddigesii. This outcome was corresponding to the classification result by using the traditional method. It is concluded that AFLP markers can be used on the studies of genetic relationships and classification of Dendrobium.