THE ROLE OF 9-cisRA IN THE REGULATION OF PIG PREADIPOCYTE DIFFERENTIATION
LIN Ya Qiu ZHUANG He Lin YANG Gong She (Laboratory of Animal Fat Deposition and Muscle Development, Northwest A & F University, Shaanxi Yangling 712100, China)
RT-PCR,Oil red O staining,Oil red O staining extract and spectrometer were used to study the effect and mechanisms of different concentrations of 9-cis RA (0-10 μmol/L) on the differentiation of pig primary preadipocyte. The results showed that different concentrations of 9-cis RA had different effect on the differentiation of preadipocyte. Low concentrations of 9-cisRA (0-10 nmol/L)could promote the differentiation of preadipocyte and upregulate the expression of RXRα,PPARγ mRNA and the activity of GPDH. High concentrations of 9-cisRA (100nmol/L-10μmol/L) could inhibit the differentiation of preadipocyte and downregulate the RXRα,PPARγ mRNA and the activity of GPDH. These results showed that different concentrations of 9-cis RA could promote or inhibit the differentiation of pig preadipocyting by regulating the expressions of RXRα and PPARγ.