CLONING AND BIOINFORMATIC ANALYSIS OF THE cDNA ENCODING A NOVEL HEAT-SHOCK FACTOR OsHSF13 IN ORYZA SATIVE L.
WU Li Juan1,2 ZHANG Zhen1 PENG Ri He2 XIONG Ai Sheng1,2 LIU Jin Ge1,2 FU Xiao Yan1,2 GAO Feng2,3 YAO Quan Hong2 (1Department of Horticulture,Nanjing Agricultural University, Nanjing 210095; 2Biotechnology Research Institute,Shanghai Academy of Agricultural Sciences, Shanghai 201106; 3College of Bioscience and Biotechnology, Yangzhou University Jiangsu, Yangzhou 225009)
As a crucial transcription factor family,heat-shock factors were mainly analyzed and characterized in tomato and Arabidposis. In this study,we isolated a putative heat-shock factor OsHSF13 that interacted specifically with heat-shock element(HSE) from Oryza sativa L. by yeast one-hybrid method. The full-length cDNA of OsHSF13 has a 1047bp open reading frame(ORF). It has HSF special DNA-binding domain(DBD) and oligomerization domain(OD). Analysis of the deduced amino acid sequences revealed that OsHSF13 was a class C heat shock factor(HSF) with all the conserved sequence elements characteristic of heat stress transcription factor. Bioinformatic analysis showed that the sequence and structure of OsHSF13′s DNA binding domain had a high similarity with LpHSF24.