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YING Xue Xiang~1 ZOU Qiang~(1**) XU Yong Hua~(2**) WANG En Zhong~2 WANG Hong Ying~1 HU Yi Hong~2 NI Quan Xing~1 (~1Department of General Surgery,Huashan Hospital,Fudan University,Shanghai 200040,China;~2Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China)  
To study DCIK co-culture system with Her-2 as the pulsing antigen peptide of DC and the cytotoxicity of DCIKs against the breast cancer cells,CIK and DC were first induced and isolated from human peripheral blood.CIK and DC pulsed with antigen Her-2 peptide were then co-cultured to prepare CIK-DC co-culture system(DCIK-P).Using DCIK-P as effect cells and breast tumor cells(MDA-MB-231,SK-BR-3,MCF-7)as target cells,the antigen-specicfic cytotox- icity of DCIK-P was analysed by cell viability and cytotoxicity assay.The killer activity of DCIKs against the MDA-MB-231,SK-BR-3 and MCF-7 is 50.38%±3.25%,52.19%±3.25% and 47.09%±2.41% respectively.The killer activity of DCIK-Ps against the MDA-MB-231,SK-BR-3 and MCF-7 is 76.30%±1.74%(P0.001),55.70%±3.05%(P=0.014) and 47.67%±2.40%(P=0.697) respec- tively.The killer activity of DCIK-P cells was greatly enhanced against Her-2(+) breast tumor cell strains.The present results can provide tumor immunological data and experimental techniques for the development of breast tumor treatment.
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