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THE INFLUENCE OF ELICITOR ON THE DISTRIBUTION PATTERN OF MICROTUBULE AND THE CYTOSOLIC CALCIUM IN MESOPHYLL PROTOPLAST OF WHEAT

LIU Gang~1 DANG Lei~2 WANG Dong Mei~(1*) (~1College of Life Science,Agricultural University of Hebei,Baoding 071001;~2Callege of Life Science,University of Zhejiang,Hangzhou 310058)  
By using immunofluorescence labeling technique and incubating the Fluo-3 AM of Ca~(2+) fluorescein,wheat(Triticum aestivum) cultivars Lovrin 10 and 5389 were selected as mesophyll pro- toplast material interaction with elicitor in this paper for studying the relationship between the pattern of microtubule and [Ca~(2+)]_(cyt).Confocal Laser Scanning Microscopy(CLSM) was used and pharmacologic examination was set.The results indicated:when treated with elicitor,the cytosolic calcium in the protoplasts from the Lovrin 10 was increasing and the pattern of microtubule showed depolymerization. In the protoplasts which MTs were depolymerized by oryzalin prior to elicitor treatment,the increase of cytosolic calcium was even higher than that in the one which was not depolymerized.
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