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EFFECTS OF SHOCK LYMPH INDUCING THE MESENTERY MICRO-LYMPHATIC ENDOTHELIAL CELLS APOPTOSIS OF RATS

NIU Chun Yu~1 ZHAO Zi Gang~1 CHEN Rui Hua~1 LI Ji Cheng~2 ZHANG Jing~1 LIU Yan Kai~1 ZHANG Yu Ping~1 (~1Department of Pathophysiololgy,Hebei North University,Zhangjiakou 075029,China;~2Institute of Cell Bioloby,Zhejiang University,Hangzhou 310003,China)  
The model of serious hemorrhagic shock was estabhshed in the condition of asepsis and mesentery lymph was taken out.As control,normal mesentery lymph fluid,normal portal vein blood and shock portal vein blood of rats were taken out.The primary mesentery micro-lymphatic endothelial ceils (MMLECs) of passages 3 were treated by different treatment factors,respectively.The cells prolif- eration by shock lymph and normal lymph of different final concentrations was measured using MTT method.The cell cycle arrest was analyzed by flow cytometry,and the electrophoresis analyze on DNA of cell nucleus was observed.The expressions of relative genes of apoptosis such as fas,fasL,bcl-2 and bax of MMLECs were detected by RT-PCR The results showed that the MMLECs proliferation was decreased when the concentration of shock lymph increased to some extent and showed statisticly sig- nificant difference compared with normal lymph group.The cells cocultured with shocking lymph fluid at 4% final concentration showed that apoptosis rate of MMLECs was (35.4±1.6)%,and G_0-G_1 cell pop- ulation was higher and the proportion of S+G_2-M cell population was lower than that of other groups, and the DNA ladder was observed in electrophoresis of cell nucleus DNA at same time.And the ex- pressions level of fas,fasL and bax mRNA were higher and bcl-2 mRNA was lower in shock lymph group than that of control group.The results demonstrated that shock lymph could reduce the cells proliferation,interfere with cell cycle,and accelerate high expression of apoptosis accelerative genes. As a result,shock lymph could induce the MMLECs apoptosis.
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