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GENETIC EVALUATION OF TOXICODENDRON VERNICIFLUUM CULTIVARS USING AMPLIFIED FRAGMENT LENGTH POLYMORPHISM MARKERS

ZHAO Xi Ping WEI Shuo Nan~(**) (Key Laboratory of Resource Biology and Biotechnology in Western China-Ministry of Education,Northwest University,Xi'an 710069,China)  
Accurate identification of Toxicodendron vernicifluum varieties and understanding their genetic relationships are essential for the improved varieties selection.In this study,Amplified fragment length polymorphism(AFLP)markers was used to evaluate the genetic resources of nine Toxicodendron vernicifluum cultivars from shaanxi.Eight pairs of AFLP primers with MseⅠfluores- cent labeled were screened from EcoRⅠ/MseⅠprimer combinations.Different pair of primers differs widely to detect genetic polymorphism of Toxicodendron vernicifluum.The polymorphic bands detect- ed by each pair of primers ranged from 242 to 622 with average value of 350.88.Eight pairs of AFLP primers produced 4463 bands in total.Among them,2807 bands (62.64%) were polymorphic. genetic similarity coefficient (SC) among all cultivars varied from 0.673 to 0.833,which further veri- fied that only a little genetic diversity existed among the cuhivars.It was the first time that AFLP technique was applied to identify Toxicodendron vernicifluum cultivars.The study suggested that AFLP technique is a useful tool for individual identification of Toxicodendron vernicifluum cultivars and could be applied to the Toxicodendron vernicifluum'fingerprinting work in China.
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