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DIFFERENT PROTEINS IN MITOCHONDRIAL PROTEOME OF T-TYPE MAIZE CYTOPLASMIC MALE-STERILE LINE AND ITS MAINTAINER LINE

ZHANG Wei Jia LI Chun Zheng HUANG Hai Quan WANG Zhen Ying~(**) PENG Yong Kang~(**) (Department of Biology,Tianjin Normal University,Tianjin 300387)  
Using T-type maize cytoplasmic male sterile line(T-CMS)and maintainer line as experimental materials,we separated mitochondrial proteins from leaves at seedling,shooting, booting stages,mesocotyl,root and anther at meiosis of pollen mother cell,single-double nucleus stage of pollen grain by two-dimensional electrophoresis with immobilized pH3-10 gradients.About 150 mitochondrial protein spots in seedling leaves,150 spots in mesocotyls,150 spots in roots and 100 spots in meiosis anther were observed respectively in this investigation.6 difference pro- tein spots were identified by MALDI-TOF-MS analysis and NCBI database searching,r40cl protein was present in mesocotyl of T-CMS and absent in maintainer line.Mature anther-specific protein, DNA-directed RNA polymerase 23kDa subunit,hexokinaseⅡwere present and glutathione S- transferase,putative polyprotein were absent in pollen aborted anther of T-CMS.Developmental changes in mitochondrial proteins were found in leaves but no differences were observed in T-CMS and its maintainer line.Obvious differences of mitochondrial proteins were found at single-double nucleus stage anther in T-CMS and maintainer line.These different proteins were considered to be associated to pollen aborted in T-CMS.
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