ANTHER DEVELOPMENT AND SPERM ISOLATION OF PSEUDOSTELLARIA HETEROPHYLLA(Miq.)
YE Zu Yun~(1,2) DENG Hua~1 JIAN Ming Xia~1 ZHENG Song~1 TIAN Hui Qiao~(1**) (~1School of Life Sciences,Xiamen University,Xiamen 361005; ~2Department of Biology,Ningde Teachers College,Ningde Fujian 352100)
Anther wall is general and tapetum is glandular.The process of meiosis of mi- crospore mother cells is simultaneous and the tetrads are tetrahedral.The mature pollen of Pseu- dostellaria heterophylla(Miq.) is tree-celled.There are 22-30 germ pores on the pollen wall. Many pollen grains could burst in 10% mannitol or 15% sucrose solution and release a pair of sperm cells which could keep alive for 25-50 min by FDA fluorescence.Using micromanipulator the released sperm cells could be collected.When pollen grains were put into a solution containing 0.03% CaCI2,0.01% H_3BO_3,0.01% KH_2PO_4 and 20%PEG for 2-5 min,they would germinate and the pollen tubes would reach 815μm at 2h after cultured.A pair of sperms would enter into pollen tube when it grew to 500-600μm.The fluorescence of both sperms would be observed clearly in pollen tube after DAPI staining.When the pollen tubes were burst in a bursting solu- tion,a pair of sperms would be released from pollen tube.